ALERT

In observance of the upcoming holidays, Agilent Cross Lab iLab Operations Software will be closed during US hours on Monday December 25th, 2017 and Monday January 1st, 2018. EU and APAC will be closed on Tuesday December 26th, 2017. During the week, we will have limited support hours and resume regular support hours on Tuesday January 2nd, 2018. For urgent matters, please add "URGENT" to the ticket subject and these requests will be prioritized first.

 

 

To submit a help ticket with iLab, click on the HELP link to access the support portal. If you are unable to access the support portal, email ilab-support@agilent.com and this will create a ticket. iLab Support can also provide you with access to the support portal.

Arrow refresh small

UTHealth Human Genetics Center Laboratory

About Us

The Human Genetics Center (HGC) was established as a research center within the School of Public Health in September of 1994 at the University of Texas Health Science Center at Houston (UTHealth). It represents the consolidation of two long-standing research Centers that have been joint enterprises of the Graduate School of Biomedical Sciences and the School of Public Health. The HGC Laboratory utilizes the latest genomic technology to understand the etiology of many common chronic diseases in human populations and uses contemporary scientific methods to facilitate the progress of future genomic research which includes elucidation of the interactions between genes and environment and translation of these findings into precision medicine initiatives to help guide decisions regarding the prevention, diagnosis, and treatment of disease. (Photo above by Nash Baker.)

 

Dr. Eric Boerwinkle is Dean of the School of Public Health and Director of the Human Genetics Center Laboratory which has more than 20 years of experience in large collaborative epidemiological studies involving the determination of human genetic variation. The HGC Laboratory has all necessary equipment needed to carry out current methodologies in molecular genetics research and is experienced in many methods for genotyping and epityping spanning low to high throughput capacities that can be tailored to meet your individual project goals. DNA and RNA extraction, aliquoting and management of hundreds of thousands of samples from several large population-based projects are routinely performed.

 

Dr. Alanna Morrison is Director of the Human Genetics Center and tenured Professor and Chair of the Department of Epidemiology, Human Genetics, and Environmental Sciences at The University of Texas School of Public Health. She is trained in statistical genetics and has expertise in genomic studies of various disease endpoints. Dr. Morrison has carried out several large-scale genomic studies, including genome-wide association studies (GWAS) and evaluation of population-based re-sequencing data. She has lead the analysis of genomic data, including association studies involving HapMap and 1000 Genomes imputed variation, exome sequence, Illumina HumanExome v1.0 BeadChip (“exome chip”) array data, and whole genome sequence.

 

Data Management:  The HGC Laboratory has developed and uses an in-house Laboratory Information Management system (LIMS) for accurate sample tracking and data collection. Data transfer is accomplished using a client-server computing system that integrates specialized laboratory instrumentation, custom client applications, barcode scanning devices, and high-capacity database server technology. Sample inventory and freezer storage is managed using in-house custom applications that track sample attributes, monitor laboratory activities and store inventory data to SQL Server databases. All samples and reagents are bar-coded, and each piece of equipment has bar-code readers. Data is backed-up nightly and tape backups are stored off-site for safe-keeping.

 

Biorepository Facilities:  Long term liquid nitrogen and ultra-low freezer storage facilities are available for safe storage of biological specimens. All freezers are connected to a call-down alarm system and personnel are available at all times to respond to emergencies.

 

Genotyping and Epityping Expertise:  The HGC Core Laboratory has more than two decades of experience and we have extensive knowledge with low and high throughput genotyping technologies, including Illumina Infinium arrays, Life Technologies TaqMan and Open Array, Agena Biosciences iPlex MassARRAY)and EpiTYPER, and Pyrosequencing genotyping platforms. The HGC Laboratory is fully automated and utilizes a suite of robotic liquid handlers, including the Tecan EVO, Hamilton Starlet and several Beckman robotic workstations. The HGC Core Laboratory has processed hundreds of thousands of samples to date using Illumina Infinium chemistry from both gDNA and samples derived from formalin-fixed, paraffin-embedded (FFPE) tissue which have resulted in publications in top tier journals (see references below); including a large Epigenome Wide Association Study of African Americans (Demerath et al., 2015) and a large genetic array project which comprised >62,000 samples (Grove et al., 2013). The HGC Core Laboratory serves as the NHLBI-contract facility for the Atherosclerosis Risk in Communities (ARIC) study, Trans-Omics for Precision Medicine (TOPMed) Centralized Omic REsource (CORE), and is collaborating with other cohorts, such as the Framingham Heart Study (FHS), Cardiac Arrest Blood Study (CABS), Genetic Epidemiology Network of Arteriopathy (GENOA) study, Coronary Artery Risk Development in Young Adults Study (CARDIA), and pediatric leukemia cancer survivors with the aim of understanding or identifying a novel, modifiable molecular basis for disease risk. The Core also participates in multi-institutional collaborations such as the Cohorts for Hearth and Aging Research in Genomic Epidemiology (CHARGE) Epigenetics working group to stay abreast of technical and analytical improvements in working with genome-wide genetic and methylation data and has the capacity to perform follow up measurements or validation of significantly associated CpG sites using EpiTYPER (Agena Bioscience; San Diego, CA).

 

Overview of Services

Lab

Illumina Whole Genome and Consortium Arrays

Whole-genome genotyping provides an overview of the entire genome, enabling genome-wide discoveries and associations. The following arrays are routinely used, but there are many Illumina arrays not listed which can be easily performed.

  • Global Screening Array
    • ~660,000 fixed markers; up to 50,000 custom marker add-on capacity
  • HumanOmni2.5-8
    • >2.3 million fixed markers; up to 200,000 custom marker add-on capacity
  • Human Omni2.5Exome
    • >2.6 million fixed markers
  • HumanOmniExpress
    • ~710,000 fixed markers; up to 30,000 custom marker add-on capacity
  • Human Exome
    • 243,345 fixed markers; up to 400,000 custom marker add-on capacity
  • HumanCoreExome
    • 551,839 fixed markers; up to 100,000 custom marker add-on capacity
  • MEGAEX  Array (formerly African Diaspora_PowerChip)
    • >1.4 million fixed markers; up to 245,000 custom marker add on capacity
  • MEG Array
    • >1.7 million fixed markers; up to 245,000 custom marker add-on capacity

Custom Mid to High-Plex Genotyping

  • Illumina iSelect Beadchips
    • Design a custom genotyping panel that supports up to 1,000,000 custom markers (SNPs, indels, and CNVs). iSelect can be deployed on either the 24-sample HD (3,072 to 90,000), 24-sample HTS (90,001 to 700,000), or other BeadChip format to support greater than 700,000 markers. Creation of these custom assays enables focused, high-throughput genotyping applications tailored to your unique project needs.

Custom Low to Mid-Plex Genotyping

  • Life Technologies TaqMan Allelic Discrimination Assays
    • Applied Biosystems (AB) TaqMan Assay provides a fast and simple way to get single nucleotide polymorphism (SNP) genotyping results. Each TaqMan SNP Genotyping Assay includes two allele-specific TaqMan probes containing distinct fluorescent dyes and a PCR primer pair to detect specific SNP targets.
  • Agena Bioscience MassARRAY
    • Applied Biosystems (AB) TaqMan Assay provides a fast and simple way to get single nucleotide polymorphism (SNP) genotyping results. Each TaqMan SNP Genotyping Assay includes two allele-specific TaqMan probes containing distinct fluorescent dyes and a PCR primer pair to detect specific SNP targets.

Epigenetics: Methylation Analysis

  • Illumina Methylation EPIC Array
    • MethylationEPIC BeadChip offers a unique combination of comprehensive, expert-selected coverage, including 99% of RefSeq genes, 95% of CpG islands, ENCODE enhancer regions, and other content categories selected by methylation experts, making it ideal for epigenome-wide association studies (EWAS).
  • EpiTYPER DNA Methylation
    • Agena Bioscience’s EpiTYPER DNA methylation analysis technology allows you to interrogate tens to hundreds of CpGs in amplicons of up to 600 bp and detect down to 5% differences in methylation.

 

Illumina Laboratory Best Practices and Quality Control

     Genotyping laboratory best practices will be followed using established Illumina protocols (www.illumina.com) and as previously described in Grove et al. It has been well documented that the GenCall algorithm used by Illumina’s GenomeStudio software has difficulty accurately detect and assigning genotype calls of rare variants (MAF < 0.01) (Korn et al., 2008; Ritchie et al., 2011). In order to capture these missed rare variants, we will perform additional post processing steps which include using zCall v3.4_GenomeStudio (Goldstein et al., 2012) to statistically determine genotypes of missing calls. The original dataset from GenomeStudio (with missing calls) will then be compared to the zCalled dataset, and a list of variants in which the heterozygote count is different between the two datasets will be manually reviewed for accuracy and calling. The final dataset will then be converted to PLINK (Purcell et al., 2007) and returned to the provider in a analysis-ready format.

 

Genomic Data Analysis and Quality Control

***Please inquire to set up a consultation to discuss your project needs.

 

Sample Handling and Storage

 

FFPE Repair
Formalin-Fixed, Paraffin-Embedded (FFPE)-derived DNA can be used by first repairing degraded DNA using the Infinium HD FFPE DNA Restore Kit from Illumina.

 

Bisulfite Conversion
The EZ-96 DNA Methylation™ Kit from Zymo Research is used to convert cytosine to uracil before methylation array analyses. This is the required kit recommended by Illumina for use with their arrays.

 

DNA extraction
Gentra Puregene Blood Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references. Gentra Puregene Blood Kits use approximately 1.5 mL of starting material (buffy coats, saliva, serum, and umbilical cord blood) and yield roughly 200-400 µg of gDNA, but can be scaled to individual project needs.

 

RNA extraction
The RNA isolation from PAXgene blood samples is conducted with the MagMAX for Stabilized Blood Tubes RNA Isolation Kit provided by Life Technologies. This kit is ideal for PCR, RT-PCR and RNA sequencing applications. The kit uses the magnetic-based purification components to isolate RNA and yields are routinely ≥3 μg from 2.5 mL of human whole blood.

GLOBINclear™-Human Kits are used to deplete >95% of the alpha and beta globin mRNA from total RNA preparations derived from whole blood for samples that will undergo future sequencing. Globin removal allows for detection of up to 50% more genes and achieves greater representation of the genome.

 

 

References

 

  1. Peloso, G.M., Auer, P.L., Bis, J.C., Voorman, A., Morrison, A.C., Stitziel, N.O., Brody, J.A., Khetarpal, S.A., Crosby, J.R., Fornage, M., et al. (2014). Association of low-frequency and rare coding-sequence variants with blood lipids and coronary heart disease in 56,000 whites and blacks. Am J Hum Genet 94, 223-232.
  2. Wessel, J., Chu, A.Y., Willems, S.M., Wang, S., Yaghootkar, H., Brody, J.A., Dauriz, M., Hivert, M.F., Raghavan, S., Lipovich, L., et al. (2015). Low-frequency and rare exome chip variants associate with fasting glucose and type 2 diabetes susceptibility. Nature communications 6, 5897.
  3. Lunetta, K.L., Day, F.R., Sulem, P., Ruth, K.S., Tung, J.Y., Hinds, D.A., Esko, T., Elks, C.E., Altmaier, E., He, C., et al. (2015). Rare coding variants and X-linked loci associated with age at menarche. Nature communications 6, 7756.
  4. Huffman, J.E., de Vries, P.S., Morrison, A.C., Sabater-Lleal, M., Kacprowski, T., Auer, P.L., Brody, J.A., Chasman, D.I., Chen, M.H., Guo, X., et al. (2015). Rare and low-frequency variants and their association with plasma levels of fibrinogen, FVII, FVIII, and vWF. Blood 126, e19-29.
  5. van der Lee, S.J., Holstege, H., Wong, T.H., Jakobsdottir, J., Bis, J.C., Chouraki, V., van Rooij, J.G., Grove, M.L., Smith, A.V., Amin, N., et al. (2015). PLD3 variants in population studies. Nature 520, E2-3.
  6. Aslibekyan, S., Demerath, E.W., Mendelson, M., Zhi, D., Guan, W., Liang, L., Sha, J., Pankow, J.S., Liu, C., Irvin, M.R., et al. (2015). Epigenome-wide study identifies novel methylation loci associated with body mass index and waist circumference. Obesity (Silver Spring) 23, 1493-1501.
  7. Hagg, S., Ganna, A., van der Laan, S.W., Esko, T., Pers, T.H., Locke, A.E., Berndt, S.I., Justice, A.E., Kahali, B., Siemelink, M.A., et al. (2015). Gene-Based Meta-Analysis of Genome-Wide Association Studies Implicates New Loci Involved in Obesity. Hum Mol Genet.
  8. Yu, B., Zheng, Y., Alexander, D., Morrison, A.C., Coresh, J., and Boerwinkle, E. (2014). Genetic determinants influencing human serum metabolome among African Americans. PLoS Genet 10, e1004212.
  9. Franceschini, N., Hu, Y., Reiner, A.P., Buyske, S., Nalls, M., Yanek, L.R., Li, Y., Hindorff, L.A., Cole, S.A., Howard, B.V., et al. (2014). Prospective associations of coronary heart disease loci in African Americans using the MetaboChip: the PAGE study. PLoS One 9, e113203.
  10. Carty, C.L., Bhattacharjee, S., Haessler, J., Cheng, I., Hindorff, L.A., Aroda, V., Carlson, C.S., Hsu, C.N., Wilkens, L., Liu, S., et al. (2014). Analysis of metabolic syndrome components in >15 000 african americans identifies pleiotropic variants: results from the population architecture using genomics and epidemiology study. Circ Cardiovasc Genet 7, 505-513.
  11. Lin, D.Y., Tao, R., Kalsbeek, W.D., Zeng, D., Gonzalez, F., 2nd, Fernandez-Rhodes, L., Graff, M., Koch, G.G., North, K.E., and Heiss, G. (2014). Genetic association analysis under complex survey sampling: the Hispanic Community Health Study/Study of Latinos. Am J Hum Genet 95, 675-688.
  12. Gong, J., Schumacher, F., Lim, U., Hindorff, L.A., Haessler, J., Buyske, S., Carlson, C.S., Rosse, S., Buzkova, P., Fornage, M., et al. (2013). Fine Mapping and Identification of BMI Loci in African Americans. Am J Hum Genet 93, 661-671.
  13. Wu, Y., Waite, L.L., Jackson, A.U., Sheu, W.H., Buyske, S., Absher, D., Arnett, D.K., Boerwinkle, E., Bonnycastle, L.L., Carty, C.L., et al. (2013). Trans-ethnic fine-mapping of lipid loci identifies population-specific signals and allelic heterogeneity that increases the trait variance explained. PLoS Genet 9, e1003379.
  14. Scott, R.A., Lagou, V., Welch, R.P., Wheeler, E., Montasser, M.E., Luan, J., Magi, R., Strawbridge, R.J., Rehnberg, E., Gustafsson, S., et al. (2012). Large-scale association analyses identify new loci influencing glycemic traits and provide insight into the underlying biological pathways. Nat Genet 44, 991-1005.
  15. Locke, A.E., Kahali, B., Berndt, S.I., Justice, A.E., Pers, T.H., Day, F.R., Powell, C., Vedantam, S., Buchkovich, M.L., Yang, J., et al. (2015). Genetic studies of body mass index yield new insights for obesity biology. Nature 518, 197-206.
  16. Spencer, K.L., Malinowski, J., Carty, C.L., Franceschini, N., Fernandez-Rhodes, L., Young, A., Cheng, I., Ritchie, M.D., Haiman, C.A., Wilkens, L., et al. (2013). Genetic variation and reproductive timing: African American women from the Population Architecture using Genomics and Epidemiology (PAGE) Study. PLoS One 8, e55258.
  17. Buyske, S., Wu, Y., Carty, C.L., Cheng, I., Assimes, T.L., Dumitrescu, L., Hindorff, L.A., Mitchell, S., Ambite, J.L., Boerwinkle, E., et al. (2012). Evaluation of the metabochip genotyping array in African Americans and implications for fine mapping of GWAS-identified loci: the PAGE study. PLoS One 7, e35651.
  18. Avery, C.L., Sethupathy, P., Buyske, S., He, Q., Lin, D.Y., Arking, D.E., Carty, C.L., Duggan, D., Fesinmeyer, M.D., Hindorff, L.A., et al. (2012). Fine-mapping and initial characterization of QT interval loci in African Americans. PLoS Genet 8, e1002870.
  19. Hiltunen, T.P., Donner, K.M., Sarin, A.P., Saarela, J., Ripatti, S., Chapman, A.B., Gums, J.G., Gong, Y., Cooper-DeHoff, R.M., Frau, F., et al. (2015). Pharmacogenomics of hypertension: a genome-wide, placebo-controlled cross-over study, using four classes of antihypertensive drugs. Journal of the American Heart Association 4, e001521.
  20. Demerath, E.W., Guan, W., Grove, M.L., Aslibekyan, S., Mendelson, M., Zhou, Y.H., Hedman, A.K., Sandling, J.K., Li, L.A., Irvin, M.R., et al. (2015). Epigenome-wide association study (EWAS) of BMI, BMI change and waist circumference in African American adults identifies multiple replicated loci. Hum Mol Genet 24, 4464-4479.
  21. Grove, M.L., Yu, B., Cochran, B.J., Haritunians, T., Bis, J.C., Taylor, K.D., Hansen, M., Borecki, I.B., Cupples, L.A., Fornage, M., et al. (2013). Best practices and joint calling of the HumanExome BeadChip: the CHARGE Consortium. PLoS One 8, e68095.
  22. Korn, J.M., Kuruvilla, F.G., McCarroll, S.A., Wysoker, A., Nemesh, J., Cawley, S., Hubbell, E., Veitch, J., Collins, P.J., Darvishi, K., et al. (2008). Integrated genotype calling and association analysis of SNPs, common copy number polymorphisms and rare CNVs. Nat Genet 40, 1253-1260.
  23. Ritchie, M.E., Liu, R., Carvalho, B.S., and Irizarry, R.A. (2011). Comparing genotyping algorithms for Illumina's Infinium whole-genome SNP BeadChips. BMC bioinformatics 12, 68.
  24. Goldstein, J.I., Crenshaw, A., Carey, J., Grant, G., Maguire, J., Fromer, M., O'Dushlaine, C., Moran, J.L., Chambert, K., Stevens, C., et al. (2012). zCall: a rare variant caller for array-based genotyping. Bioinformatics 28, 2543-2545.
  25. Purcell, S., Neale, B., Todd-Brown, K., Thomas, L., Ferreira, M.A., Bender, D., Maller, J., Sklar, P., de Bakker, P.I., Daly, M.J., et al. (2007). PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet 81, 559-575.

Leadership

Eric Boerwinkle, PhD, Dean UTHealth School of Public Health; Professor of Epidemiology, Human Genetics and Environmental Sciences 

Alanna C. Morrison, PhD, FAHA, Professor and Department Chair, Department of Epidemiology, Human Genetics and Environmental Sciences; Director, Human Genetics Center

Megan L. Grove, MS, Principal Laboratory Specialist; Associate Director, Human Genetics Center Laboratory

Location and Hours of Operation

UTHealth Human Genetics Center Laboratory

1200 Pressler Street

Houston TX 77030

713-500-9800

8:00 a.m. - 5:00 p.m. (CT)

Contacts

Name Role Phone Email Location
Megan Grove, MS
Principal Laboratory Specialist; Associate Director
 
713-500-9800
 
HGC_lab@uth.tmc.edu
 

 

Price List


Search available services:
Name Description Price
Data Analysis Inquire

Map



Members at UT System

Core Name Primary Contact Email Phone Number/Ext
MDACC AIM

<p>AIM&nbsp;provides assistance to researchers with collection and assessment of Patient Reported Outcomes, behavioral data, and measures of fitness and body composition.</p>

Susan K. Peterson, PhD, MPH speterso@mdanderson.org 713-792-8267
MDACC Bionutrition Research Core

Christine Ranieri, MS, RD, LD brc@mdanderson.org 713-794-1295
MDACC Biospecimen Extraction Facility

The Biospecimen Extraction Resource provides a centralized laboratory for standardized, high quality DNA, RNA and protein extraction from blood, tissues, saliva, cells, or other patient derived biospecimens.

Jian Gu, Ph.D. jiangu@mdanderson.org 713-792-8016
MDACC Bone Histomorphometry Core Laboratory

The Bone Histomorphometry Core Laboratory provides histomorphometric analyses of nondecalcified bone specimens, including processing mouse bone specimens for nondecalcified histology and performing static and dynamic histomorphometric analyses of mouse bone specimens.

Michael Starbuck mwstarbu@mdanderson.org 713-563-1212
MDACC Characterized Cell Line Core Facility

The Characterized Cell Line distributes well characterized and validated cell lines to researchers across the world and offers cell line validation using short tandem repeats STR profiling.

Kathryn Aziz ccsgcclc@mdanderson.org 713-792-5743
MDACC Chemical Imaging Research Core

Dodge Baluya DLBaluya@mdanderson.org 713-794-1661
MDACC Core for Biomolecular Structure and Function (CBSF)

The purpose of the Core for Biomolecular Structure and Function (CBSF) is to provide recombinant &nbsp;protein production, protein characterization, binding studies and structural biology (X-ray crystallography)&nbsp;services to MD Anderson Cancer Center and the wider Texas Medical Center.

Paul Leonard PLeonard1@mdanderson.org 832-750-1516
MDACC DNA Methylation Core

The DNA Methylation Core is funded by the Center for Cancer Epigenetics and provides services related to the analysis of DNA methylation for researchers at the University of Texas M.D. Anderson Cancer Center.

Marcos Estecio mestecio@mdanderson.org 713-792-9108
MDACC Gene Editing/Cellular Model Core Facility

The Gene Editing Cellular Model Core Facility provides custom cellular model construction services using two technical platforms.

Dr. Junjie Chen jchen8@mdanderson.org 713-792-4863
MDACC Genetically Engineered Mouse Facility (GEMF)

The Genetically Engineered Mouse Facility provides unique models with wide application to basic and translational research. The GEMF supplies indispensable expertise to generate these models in an efficient and cost effective manner using state of the art procedures.

Jan Parker-Thornburg, Ph.D. jpthorn@mdanderson.org 713-745-2654, 713-745-1419 (lab)
MDACC High Resolution Electron Microscopy Facility (HREMF)

The mission of the High Resolution Microscopy Facility is to provide scanning and transmission electron microscopy services to researchers.

Robert R. Langley, Ph.D. rlangley@mdanderson.org 713-792-9142, 713-792-8747 (fax)
MDACC Laboratory Animal Genetic Services

The Laboratory Animal Genetic Service Core provides services for speed congenics, background characterization, microbial PCR testing, rodent STR and murine genetic consultations.

Fernando Benavides, DVM, PhD, DACLAM fbenavid@mdanderson.org 512-237-9343
MDACC Molecular Cytogenetics Core Facility

The primary goal of this facility is to offer conventional and molecular cytogenetic services, including species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping

Asha S. Multani Ph.D. amultani@mdanderson.org 713-563-1892
MDACC Monoclonal Antibody Core Facility

The Monoclonal Antibody Facility develops high quality, cost effective customized MAbs to meet the basic, translational, and clinical research needs of MD Anderson investigators.

Laura Bover lbover@mdanderson.org 713-563-3301 or 713-563-2281
MDACC NORTH Campus Flow Cytometry and Cellular Imaging Core Facility

The Flow Cytometry and Cellular Imaging Facility was established to provide access to state of the art cell analysis technology for researchers, and provides cell sorting, analytical flow cytometry, cellular imaging and custom monoclonal antibody mAb conjugations to its users.

Joan Hoover-Zuniga jezuniga@mdanderson.org 713-745-1749
MDACC Nuclear Magnetic Resonance Facility

The NMR facility is used for structure determination of compounds developed in drug discovery, pharmacology, and diagnostic imaging laboratories.

Kumaralal K. Kaluarachchi, Ph.D kkkaluar@mdanderson.org 713-792-3779
MDACC Pharmaceutical Chemistry Facility

The mission of the Pharmaceutical Chemistry Facility is to assist researchers with the design and synthesis of compounds for use in cancer research.

Barbara Czako Ph.D BCzako@MDAnderson.org 713-745-2050
MDACC Protein Array & Analysis Core (PAAC)

The Protein Array and Analysis Core PAAC at the University of Texas MD Anderson Cancer Center Science Park provides investigators access to innovative and cutting edge cancer research tools for the discovery and characterization of novel proteinprotein interactions.

Cari Sagum casagum@mdanderson.org 512-237-9328
MDACC Proteomics and Metabolomics

The mission of the Proteomics and Metabolomics Core Facility is to provide the research community with cutting edge expertise and services for sensitive, precise, and accurate measurement of proteins and metabolites using mass spectrometrybased technologies.p</p>

David Hawke, Ph.D dhawke@mdanderson.org 713-834-6096
MDACC RPPA-Functional Proteomics

Functional Proteomics Reverse Phase Protein Array RPPA Core provides cancer center members with access to a powerful, highthroughput, quantitative costeffective antibodybased assay to characterize basal or ligandinduced protein expression and modification, and timeresolved responses appropriat

Kathryn Aziz ccsgrppa@mdanderson.org 713-792-5743
MDACC Research Histology Core Laboratory

The Research Histology Core Laboratory includes specialized equipment for immunohistochemistry, fluorescent in situ hybridization FISH, laser capture microdissection, and tissue microarray construction.

Jacqueline Furr jfurr@mdanderson.org 713-792-3119, 713-563-0544 (fax)
MDACC Science Park Flow Cytometry and Cell Imaging Core

The Flow Cytometry and Cell Imaging Core Facility at Science Park provides cutting edge cell analysis resources. These include cell sorting and analytical flow cytometry to phenotype, quantify and isolate cells and confocal microscopy to capture high resolution images.p

Pamela Whitney - Lab pjwhitney@mdanderson.org 512-237-9427
MDACC Science Park Molecular Biology Core

The Molecular Biology Core Facility at Science Park provides investigators access to sophisticated and stateoftheart molecular techniques, services and instrumentation.

Jianjun Shen, Ph.D. jianshen@mdanderson.org 512-237-9558
MDACC Science Park NGS

The Science Park NGS facility is built around a user group of 30 cancer researchers at MD Anderson Science Park, UT Austin, and Texas State University San Marcos.

Jianjun Shen, Ph.D jianshen@mdanderson.org 512-237-9558
MDACC Science Park Research Histology, Pathology & Imaging Core

The Science Park Research Histology, Pathology Imaging Core provides investigators with expert imaging and experimental pathology support. Our staff consult with investigators to formulate an integrated imaging, pathology and histology protocol for each study.

Manu Sebastian, DVM, PhD, DACVP, DABT, DECLAM mmsebastian@mdanderson.org 512-237-9347
MDACC Sequencing and Microarray Facility (SMF)

The Sequencing and Microarray Facility provides essential genomic analysis services to support researchers in basic science, translational, and populationbased research programs

Erika Thompson, M.S. ejthomps@mdanderson.org 713-834-6381
MDACC Sequencing and ncRNA Program

The Sequencing and Noncoding RNA Program is one of an MDA institution supported shared genomic service resources providing not only instrumentation but also expertise in applications of next generation sequencing and microarray services to investigators

Chang-gong Liu, Ph.D. cgliu@mdanderson.org 713-792-2287
MDACC Small Animal Imaging Facility

<p>The Small Animal Imaging Facility provides access to imaging instrumentation and expertise that allows researchers to integrate powerful and cuttingedge imaging technologies into routine preclinical research with confidence that their scientific objectives will be achieved.</p>

Charles Kingsley charles.kingsley@mdanderson.org (713) 563-6709
MDACC shRNA and ORFeome Core

The shRNA and ORFeome core Facility makes the following libraries available to MD Anderson members human and murine shRNA libraries, human ORFeome collaboration and LentiORF libraries. The core not only provides center members with individual shRNA and ORF clones but also builds customized librar...

Dr. Sun, Yutong ysun2@mdanderson.org (713) 792-3677
MDACC siRNA Core Facility

The siRNA Core Facility at MD Anderson performs High Throughput and High Content siRNA screens to assist experimental cancer therapy studies. The cores ultimate goal is to develop relevant three dimensional models that represent the human body..

Core Name Primary Contact Email Phone Number/Ext
The Shimadzu Center for Advanced Analytical Chemistry Maciej Kukula kukula@uta.edu
The Shimadzu Center for Bio-Molecular Imaging Jeff Campbell jeff.campbell@uta.edu
The Shimadzu Institute NanoTechnology Research Center Nader Hozhabri nh@uta.edu
Core Name Primary Contact Email Phone Number/Ext
UTSA Kleberg Advanced Microscopy Center

KAMC focuses on the synthesis and characterization of nanomaterials for imaging, labels for bioassays, and active targeting for in vivo or in vitro diagnostics. The Core studies the interaction of nanoparticles with living cells for application in the targeted delivery of drugs, genes, and proteins.

Miguel Jose-Yacaman, Ph.D. miguel.yacaman@utsa.edu (210) 458-6954
UTSA Protein Biomarker Core

The RCMI Proteomics and Protein Biomarkers Core Facility is focused on capillary liquid chromatographymass spectrometry LCMS and tandem mass spectrometry LCMSMS to identify, characterize, and quantify proteins. The Proteomics Core develops novel methods, while the Protein Biomarkers Core applies ...

Stephan Bach, Ph.D. stephen.bach@utsa.edu (210) 458-4466
UTSA RCMI Biophotonics Core

The RCMI Biophotonics Core Facility provides cutting edge technology for the study and manipulation of biological samples using light, including longterm indepth imaging of live tissues and the technology to probe at the molecular level for proteinprotein interactions within live samples.

George Perry george.perry@utsa.edu (210) 458-4450

University of Texas Health Northeast

Core Name Primary Contact Email Phone Number/Ext
Core Name Primary Contact Email Phone Number/Ext
UTHealth Atomic Force Microscopy

The BioScope™ II microscope has been engineered to facilitate advanced life science research such as imaging, probing and manipulating biological systems at the nanometric scale. We can determine the micromechanical properties of both biological and non-biological samples.

Ana Maria Zaske, PhD Ana.M.Zaske@uth.tmc.edu 713-486-5418
UTHealth Biomedical Informatics Group (BIG)

We offer services to researchers, including access to clinical data and enhanced subject recruiting, and are involved in multiple local and national collaborations with other groups.

Susan Guerrero Susan.C.Guerrero@uth.tmc.edu 713-500-3926
UTHealth CCTS Biobank

The CCTS Biobank consists of a collection of human samples and related clinical and genomic data that are available for collaborative sharing with qualified researchers and owned by contributing investigators conducting research within the Texas Medical Center.

Jennifer Sanner, PhD, RN Jennifer.E.Sanner@uth.tmc.edu 713.500.2114
UTHealth Cellular Therapy Core

<p>The CTC is an FDA registered facility where tissues are processed to produce cells for clinical applications. It is focused on translating, validating and supporting IND applications at a preclinical level into clinical grade processes.</p>

Fabio Triolo, Ph.D. Fabio.Triolo@uth.tmc.edu 713-486-2542
UTHealth Center for Advanced Microscopy

<p>The Center for Advanced Microscopy provides assisted or unassisted state of the art imaging capabilities including three confocal microscopes, fluorescence lifetime imaging, live animal imaging with an IVIS Lumina, flow cytometry, and a liquid handling multiwell plate reader.</p>

Olga Chumakova, Ph.D. Olga.Chumakova@uth.tmc.edu 713-500-7495
UTHealth Flow Cytometry Service Center

<p>The Flow Cytometry Service Center provides a broad range of assisted and unassisted flow cytometry services from basic 2 color analysis to advanced 14 parameter analysis and multi population cell sorting.</p>

Alicia Blancas, Ph.D. Alicia.A.Blancas@uth.tmc.edu 713-500-2486
UTHealth Human Genetics Center Laboratory

Megan Grove, MS HGC_lab@uth.tmc.edu 713-500-9800
UTHealth Magnetic Resonance Imaging Core

The MR group provides advanced MRI techniques, including selective tissue imaging, magnetic resonance spectroscopic imaging, diffusion tensor imaging to neurological disorders, central nervous system trauma, and drug addiction both in humans and animal models.

Ponnada Narayana, Ph.D Ponnada.A.Narayana@uth.tmc.edu 713-500-7677
UTHealth NanoChemistry Service Center

The Nanochemistry Service Center provides provides custom synthesis, labeling, and chemical coupling of DNA thioaptamers and nanoparticles and large-scale, high-resolution 3D printing services.

David Volk, PhD david.volk@uth.tmc.edu 713-500-3611
UTHealth Proteomics Service Center

The CTPC services provided are designed to identify and quantitate proteins and their modifications in a broad range of research specimens from simple purified protein samples to biomarker discovery and verification in complex mixtures such as cell and tissue extracts, plasma andor other biofluids

Li Li Li.Li@uth.tmc.edu 713-500-2456
UTHealth Quantitative Genomics & Microarray Service Center

The Quantitative Genomics Microarray Core Lab can perform all techniques associated with a qPCR instrument, quantitative Meso Scale ELISAs and microarrays utilizing Illumina and Agilent arrays.

David Loose, Ph.D. David.S.Loose@uth.tmc.edu 713-500-7440
UTHealth Small Animal Cardiovascular Phenotyping Service Center

Siddharth Prakash Siddharth.K.Prakash@uth.tmc.edu 713-500-7003
UTHealth Therapeutic Antibody Core

The Therapeutic Antibody Core aims to provide statewide support and service to advance lead antibodies from academic laboratories to the stage of preclinical development.

Zhiqiang An, PhD Zhiqiang.An@uth.tmc.edu 713-500-3011
UTHealth Transgenic and Stem Cells Service Center

Eva Zsigmond, PhD eva.m.zsigmond@uth.tmc.edu 713-500-2453
Core Name Primary Contact Email Phone Number/Ext
UTHSA Bioanalytics and Single-Cell Core

Nameer Kirma kirma@uthscsa.edu (210) 562-4068
UTHSA Biobanking and Genome Analysis

Teresa L. Johnson-Pais, Ph.D. paist@uthscsa.edu 210.567.6571
UTHSA Center for Macromolecular Interactions

Eileen M. Lafer lafer@uthscsa.edu (210) 567-3764
UTHSA Flow Cytometry Core Facility

Karla Gorena moncadak@uthscsa.edu 210-567-3911
UTHSA Mass Spectrometry Laboratory

Susan Weintraub, Ph.D. weintraub@uthscsa.edu (210) 567-4043
UTHSA MicroCT Core Laboratory

Roberto Fajardo fajardor@uthscsa.edu 210-567-6176
UTHSA NMR / Screening

Kristin E. Cano, Ph.D. canok@uthscsa.edu 210-567-8706
UTHSA Optical Imaging Facility

Exing Wang wange3@uthscsa.edu 210-562-4062
UTHSA X-Ray Crystallography

John Hart pjh@biochem.uthscsa.edu 210-567-0751

University of Texas Medical Branch - Galveston

Core Name Primary Contact Email Phone Number/Ext
Biomolecular Resource Facility

William Russell, Ph.D bill.russell@utmb.edu (409) 772-3579
UTMB International Biosafety Training Center

Sharon Walters skwalter@utmb.edu 409-772-1781
UTMB Research Histopathology Core

The Research Histopathology Core is equipped with the latest available instrumentation. RHC’s instrumentation is operated by highly skilled personnel available to assist in tissue preparation, immunohistochemistry, immunofluorescence, Insitu hybridization, including assay optimization, image anal...

Gustavo Valbuena gvalbuen@utmb.edu (409) 747-0735
Core Name Primary Contact Email Phone Number/Ext
UTSW Clinical Research Unit (CRU)/Investigational Drug Service (IDS) Lisa Fleming, MPA CRUAdministration@UTSouthwestern.edu 214.648.2718
UTSW Flow Cytometry Facility David Farrar david.farrar@utsouthwestern.edu 24-648-7315
UTSW Genomics Sequencing & Microarray Core Facility Chaoying Liang or Indu Raman Chaoying.Liang@UTSouthwestern.edu or Indu.Raman@UTSouthwestern.edu 214-648- 7329 or 214-645-6071
UTSW High Throughput Screening Core Bruce Posner, Ph.D. bruce.posner@utsouthwestern.edu 214-648-0285
UTSW Metabolic Phenotyping Core Ruth Gordillo Ruth.Gordillo@UTSouthwestern.edu 214-648-3403
UTSW O'Brien Kidney Center: Animal Models Core Vincy George Vincy.George@UTSouthwestern.edu 214-648-7222
UTSW O'Brien Kidney Center: Cell Biology and Imaging Core Vincy George Vincy.George@UTSouthwestern.edu 214-648-7222
UTSW O'Brien Kidney Center: Clinical and Translational Research Core Vincy George Vincy.George@UTSouthwestern.edu 214-648-7222
UTSW O'Brien Kidney Center: Physiology Core Vincy George Vincy.George@UTSouthwestern.edu 214-648-7222
UTSW Preclinical Pharmacology Core Noelle Williams noelle.williams@utsouthwestern.edu 214-648-5072
UTSW Rodent Behavior Core Shari Birnbaum shari.birnbaum@utsouthwestern.edu 214-648-5123
UTSW Structural Biology Laboratory
UTSW Tissue Management Shared Resource Cheryl Lewis cheryl.lewis@utsouthwestern.edu 214-648-7597
UTSW Whole Brain Microscopy Facility (WBMF) Denise Ramirez, PhD denise.ramirez@utsouthwestern.edu 214-648-0203