UTHealth Flow Cytometry Service Center

Overview of Services

The Flow Cytometry Service Center has three flow cytometers and one cell sorter available to the public. The BD FACSAria II sorter and BD FACSCalibur analyzer are located in our main facility at the IMM (SRB 617) whereas the BD LSRFortessa (MSE R303) and Beckman Coulter Gallios (MSB 5.628) are located at the McGovern Medical School. Both locations have additional workstations available for post-acquisition data analysis.

These specialized multicolor cell analysis instruments allow researchers to evaluate a large number of samples in a short time frame. The current instrumentation allows simultaneous acquisition of up to 19 fluorescent signals from thousands of individual cells per second. The Flow Cytometry Service Center offers FACS acquisition and analysis, cell sorting, and consultation for experimental design, interpretation and troubleshooting.

The Flow Cytometry Service Center is committed to the continuing development of biological research and provides training, instrumentation, technical expertise, data analysis and interpretation for many IBC approved studies.

 

Services provided: 

  • Assisted and Unassisted data acquisition
  • Assisted and Unassisted cell sorting
  • Sorting for Single cell deposition (eg. 96 well plate) 
  • Post acquisition data analysis (FlowJo, Diva, Kaluza) 
  • Experimental design assistance including panel design, controls, etc. 
  • Troubleshooting
  • Training to become an unassisted users

All investigators are encouraged to meet with the service center staff to discuss the panel design, appropriate controls, technical limitations of the experiment etc. prior to scheduling instrument time.

The FCSC offers training for both new and experienced investigators. Depending on the level of prior knowledge and the intended frequency of equipment use, the training plan is tailored to the individual’s needs.

Location and hours of operation

Hours Location

Monday-Friday 9am-5pm

24/7 for trained individuals     

IMM - Sarofim Research Building

Room 617 (6th floor)

1825 Pressler Street

Houston, TX 77030 

Training Policies

Calibur, Fortessa and Gallios:

These instruments are mostly reserved for unassisted use. If you require training, please contact the Facility Manager to schedule a training date before your desired experiment time.

BD FACS Aria II:

ALL sorts/analysis are required to be assisted if you have not been previously trained by a core staff member.  If you expect to use the Aria II often (more than once a week), please inquire about training.

 

Links and Resources

Basic Training Tutorials:

https://www.thermofisher.com/us/en/home/support/tutorials.html

http://www.bdbiosciences.com/us/support/s/itf_launch

 

Spectrum Viewers:

http://www.bdbiosciences.com/us/s/spectrumviewer

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

http://www.biolegend.com/spectraanalyzer

 

Panel Building Tools:

https://www.chromocyte.com/

https://fluorofinder.com/

 

Basic Protocols:

https://www.thermofisher.com/us/en/home/references/protocols.html

https://en.bio-protocol.org

Contacts

Name Role Phone Email Location
Ville Meretoja, Ph.D.
Facility Manager/Senior Research Associate
 
713-500-2486
 
ville.meretoja@uth.tmc.edu
 
SRB 617
 
Amy Hazen, Ph.D.
Associate Director
 
713-500-3612
 
Amy.Hazen@uth.tmc.edu
 

 
Minh Vo
Financial Analyst
 
713-500-2450
 
Minh.H.Vo@uth.tmc.edu
 
SRB 205