UTHealth Flow Cytometry Service Center

Overview of Services

The IMM Flow Cytometry Service Center is located on the sixth floor of the Fayez S. Sarofim Research Building and maintains four instruments, a BD FACSAria II, a BD FACSCalibur, a Coulter FC500 and a Luminex LX200.

These specialized instruments allow researchers to evaluate a large number of samples at the cellular level in a short time frame. The Core offers FACS acquisition and analysis, cell sorting, and consultation for experimental design, interpretation and troubleshooting.

The Flow Cytometry Service Unit is committed to the continuing development of biological research and provides training, instrumentation, technical expertise, data analysis and interpretation for many IBC approved studies.

 Services provided: 

  • Assisted and Unassisted data acquisition
  • Assisted and Unassisted cell sorting
  • Sorting for Single cell deposition (ex. 96 well plate) 
  • Post acquisition data analysis (FlowJo, Diva, Kaluza) 
  • Experimental design assistance including panel design, controls, etc. 
  • Troubleshooting
  • Training to become an unassisted users

All investigators are encouraged to meet with core staff to discuss the experiment prior to scheduling to discuss panel design, appropriate controls, technical limitations, etc.

The FCSC offers training for both new and experienced investigators. Depending on the level of prior knowledge and the intended frequency of use of the equipment, the training plan is tailored to the specifically to the individual’s needs.

Location and hours of operation

Hours Location

Monday-Friday 9am-5pm

24/7 for trained individuals     

Room 617 (6th floor)

1825 Pressler Street

Houston, TX 77030 

Training Policies

BD Calibur:

If you require training, please contact to schedule a training date before your desired experiment time.

BD FACS Aria II:

ALL sorts/analysis are required to be assisted if you have not been previously trained by a core staff member.  If you expect to use the Aria II often (more than once a week), please inquire about training.

 

Links and Resources

 

Basic Training tutorials:

https://www.thermofisher.com/us/en/home/support/tutorials.html

http://www.bdbiosciences.com/us/support/s/itf_launch

 

Spectrum Viewers:

http://www.bdbiosciences.com/us/s/spectrumviewer

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

http://www.biolegend.com/spectraanalyzer

http://www.ebioscience.com/resources/fluorplan-spectra-viewer.htm

 

Panel Building tools:

https://www.chromocyte.com/

https://fluorofinder.com/

https://www.fluorish.com/

 

Basic Protocols:

http://www.ebioscience.com/resources/best-protocols/flow-cytometry-protocols.htm

http://www.bio-protocol.org/fllist.aspx?fl3=509&weekcount=10

https://www.lifetechnologies.com/us/en/home/references/protocols/cell-and-tissue-analysis/flow-cytometry-protocol.html

 

No Lyse Whole Blood:

https://www.miltenyibiotec.com/~/media/Files/Support/Macs-Technology/AP_MACSQuant_WholeBlood_NoLyse.ashx

 

Viability:

http://www.uvm.edu/medicine/flowcytometry/?Page=viability.html&SM=protocolsubmenu.html

http://www.researchgate.net/post/What_is_the_right_DAPI_concentration_to_use_in_a_flow_cytometry_experiment

http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/flow-cytometry/cell-health-and-viability-assays-for-flow-cytometry/cell-viability-assays-for-flow-cytometry.html

http://info.ebioscience.com/bid/58846/Flow-Cytometry-Discriminate-Between-Live-and-Dead-Cells

http://www.ebioscience.com/resources/functional-dyes-and-reagents/cell-viability-dyes.htm

http://www.ebioscience.com/media/pdf/best-protocols/viability-staining.pdf

 

Cell Cycle:

https://iacf.uchicago.edu/Protocols/DAPICellCycleProtocol.htm

http://cancer.ucsd.edu/research-training/shared-resources/flow-cytometry/protocols/Pages/cell-cycle-with-pi.aspx

http://www.abcam.com/protocols/flow-cytometric-analysis-of-cell-cycle-with-propidium-iodide-dna-staining

http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/flow-cytometry/cell-health-and-viability-assays-for-flow-cytometry/cell-cycle-assays-for-flow-cytometry.html

http://www.vet.cornell.edu/labs/cytometry/docs/DNACellCycleAnalysiswithPI.pdf

 

Apoptosis:

https://m.bdbiosciences.com/us/resources/s/annexin

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3169266/

http://www.cyto.purdue.edu/cdroms/cyto10a/educationandresearch/annexin.html

http://cancer.ucsd.edu/research-training/shared-resources/flow-cytometry/protocols/Pages/apoptosis-with-pi-only.aspx

http://www.lifetechnologies.com/us/en/home/life-science/cell-analysis/flow-cytometry/cell-health-and-viability-assays-for-flow-cytometry/apoptosis-assays-for-flow-cytometry.html

http://flowbook.denovosoftware.com/Flow_Book/Chapter_9%3A_Cell_Death,_Including_Apoptosis

 

Cytometry BOOK:

http://flowbook.denovosoftware.com/Flow_Book

http://www.coulterflow.com/bciflow/practical/book/index.html

 

Contacts

Name Role Phone Email Location
Ville Meretoja, Ph.D.
Senior Research Associate
 
713-500-2486
 
ville.meretoja@uth.tmc.edu
 
SRB 617
 
Amy Hazen, Ph.D.
Associate Director
 
713-500-3612
 
Amy.Hazen@uth.tmc.edu
 

 
Minh Vo
Financial Analyst
 
713-500-2450
 
Minh.H.Vo@uth.tmc.edu
 
SRB 205
 

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